Part:BBa_K4365018:Design

Signal peptide of HFBII from Trichoderma reesei

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimised for yeast.

Source

The sequences of the hydrophobic signal peptide was collected from literature ^[1] and was extracted via analysis of their sequence using the SignalP - 5.0 signal peptide predictor tool ^[2].

References

↑ M. G. Waters, E. A. Evans, G. Blobel (1988) Prepro-alpha-factor has a cleavable signal sequence, Journal of biological chemistry Volume 263, ISSUE 13, P6209-6214, https://doi.org/10.1016/S0021-9258(18)68773-3
↑ José Juan Almagro Armenteros et al. (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks Nature Biotechnology, 37, 420-423, doi: 10.1038/s41587-019-0036-z

[1] M. G. Waters, E. A. Evans, G. Blobel (1988) Prepro-alpha-factor has a cleavable signal sequence, Journal of biological chemistry Volume 263, ISSUE 13, P6209-6214, https://doi.org/10.1016/S0021-9258(18)68773-3

[2] José Juan Almagro Armenteros et al. (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks Nature Biotechnology, 37, 420-423, doi: 10.1038/s41587-019-0036-z

[1]

[2]